EFFECT OF HIGH BLOOD CORTISOL CONCENTRATION ON THE SPECTROSCOPICALLY DETERMINED SECOND-ARY STRUCTURE OF PROTEINS AND LIPID BALANCE ON THE EXAMPLE OF ELITE WOMEN VOLLEYBALL PLAYERS

Cortisol is a stress hormone that plays crucial role in the balance between phospholipids and lipids levels. In consequence, it affects secondary structure of proteins. Currently cortisol concentration serum determined by biochemical analysis. A new optical method to estimate level proposed this work. Infrared Raman spectroscopies were used determine quantitative qualitative changes proteins fractions function 49 samples collected from volleyball players at various stages preparation for competition. With increase, decrease structures related PO2- phospholipid groups amides III I bonds was noticed spectra. The differences absorbance visible FTIR spectra, not statistically significant. However, calculated ratios peaks vibrations lipid functional amide show, with increase concentration, amount observed transmission Pearson correlation test presented positive correlations protein levels transmittance Negative Obtained results showed, using spectroscopy effect increased on proteins, directly visible. case spectroscopy, one had calculate CH2 CH3 (2957 cm-1, 2920 2872 cm-1) (1654 cm-1), show serum. These suggest, more promising technique obtain an information about